Next Generation Sequencing (NGS) in Forensic Genetics and Anthropology Training Course

Introduction to NGS and Its Forensic Applications

• Overview of current DNA sequencing technologies
• Benefits of NGS compared to traditional forensic methods
• Standard forensic applications of NGS

Foundations of Forensic Genetics

• DNA markers utilized in forensic analysis
• Short Tandem Repeats (STRs) and Single Nucleotide Polymorphisms (SNPs)
• Mitochondrial DNA and Y-chromosome analysis

NGS Workflows in Forensic Casework

• Sample preparation and library construction processes
• Sequencing protocols tailored for forensic samples
• Quality control measures and contamination prevention strategies

Bioinformatics and NGS Data Analysis

• Processing and alignment of NGS data
• Variant calling and forensic interpretation techniques
• Software tools designed for forensic genomic analysis

Forensic Anthropology and Ancestry Determination

• Genetic markers for ancestry and population studies
• NGS applications in ancient and degraded DNA analysis
• Case studies from forensic anthropology

NGS for Challenging and Degraded Samples

• Techniques for handling low-quality and low-quantity DNA
• Managing environmental and mixed DNA samples
• NGS applications in missing persons and disaster victim identification

Legal, Ethical, and Regulatory Considerations

• Privacy issues in forensic genomics
• Regulatory frameworks for forensic DNA databases
• Ethical challenges in forensic genetic analysis

Future Trends in Forensic Genomics

• Advancements in sequencing technology
• Integration of AI in forensic genetics
• Emerging forensic applications of NGS

Summary and Next Steps